Specimen Requirements

General Specimen Requirement and Collection Instructions


  • Maintain sample sterility, keep all specimens at room temperature and deliver specimens to the laboratory within 24 - 48 hours.
  • Do not fix samples in any manner contrary to the maintenance of cell viability. If specimen collection materials (i.e., tissue culture medium, etc.) are needed, contact our laboratory prior to collecting the sample.
  • Download the complete Specimen Collection Manual (in .pdf format) here.



  • Amniotic Fluid Samples: For routine chromosome studies, aseptically collect approximately 1 cc of fluid per week of gestational age in two sterile 15 ml conical centrifuge tubes and sequentially label the tubes. The initial 1 - 2 cc should not be included for chromosome studies (but may be utilized for AFP testing). Ship samples at room temperature. Click here for a Prenatal Cytogenetic Analysis Request Form.
     
  • Blood Samples: All blood samples should be rotated thoroughly to ensure adequate mixing with the anticoagulant or preservative. Ship samples at room temperature.

    For routine chromosome studies (karyotyping) or microdeletion FISH studies, blood samples should be collected aseptically in sodium heparin tubes (usually green-top tubes). For infants, obtain approximately 2 cc, and for children and adults, obtain 5 - 7 cc.

    Note: A separate blood sample is required for CGH microarray studies.

    Click here for a Standard Cytogenetic Analysis Request Form.

    For aCGH or microarray studies, blood samples should be collected aseptically in sodium heparin tubes (usually green-top tubes). For infants obtain approximately 2 cc, and for children and adults, obtain 5 - 7 cc.

    Note: A separate blood sample is required for conventional blood chromosome and/or FISH studies. Click here for an aCGH (Microarray) Analysis Request Form

     
  • Bone Marrow Samples: Obtain 1 - 2 cc of bone marrow aspirate in a sodium heparin tube. Bone marrow samples should be rotated thoroughly to ensure adequate mixing with the anticoagulant. Ship samples at room temperature. Click here for a Oncology Cytogenetic Analysis Request Form.

     
  • Chorionic Villi Samples: Collect 10 - 30 mg of chorionic villi, utilizing aspiration medium* that contains sodium heparin. After assessment of the appropriate amount and quality of villi, transfer it to a sterile centrifuge tube with transportation medium (UF PathLabs will supply aspiration and transport mediums in separate sterile tubes upon request.).

    Click here for a Prenatal Cytogenetic Analysis Request Form.

     
  • Lymphatic Tissue Samples: Aseptically obtain a sample of approximately 2 mm x 2 mm x 2 mm and deposit it in tissue culture medium. Ship samples at room temperature.

    Click here for a Oncology Cytogenetic Analysis Request Form.

     
  • Products of Conception: As aseptically as possible, obtain tissue samples approximately 2 mm x 2 mm in size (Recommended tissues for abortuses include skin, lung and cartilage.). Transfer each sample to a separate sterile container (15 ml conical centrifuge tube), containing tissue culture medium, which has been brought to room temperature (UF PathLabs will supply these materials upon request.). Do not place the sample in cold or hot medium. To enhance the possibility of a successful cytogenetic study, we recommend that at least two tissue types be sent in separate containers.

    Click here for a Prenatal Cytogenetic Analysis Request Form.

     
  • Skin Biopsy Samples: In an aseptic manner, obtain a piece of skin approximately 2 mm x 2 mm, which is deep enough to ensure that the dermal layer is included. Deposit the specimen in a sterile container, containing tissue culture medium with antibiotics, that has been brought to room temperature. Do not immerse the sample in saline or fix it in any preservative.

     
  • Solid Tumor Samples: Aseptically obtain a sample of approximately 2 mm x 2 mm x 2 mm and deposit it in tissue culture medium. Ship samples at room temperature.

    Click here for a Oncology Cytogenetic Analysis Request Form.

Cytogenetics