Adult Islet Autoantibody Panel (ICA, GADA, IA-2A, and ZnT8A)

Methodology: 
Indirect immunofluorescence (ICA), radioimmunoassay (GADA, IA-2A), enzyme-linked immunosorbant assay (ZnT8A)
Performed: 
Weekly
Reported: 
10 - 20 business days
Use: 
  • Diagnosis of Type 1 Diabetes: The presence of islet cell cytoplasmic autoantibodies (ICA), glutamic acid decarboxylase autoantibodies (GADA), insulinoma-2 associated autoantibodies (IA-2A) and/or zinc transporter 8 autoantibodies (ZnT8A) in the serum of patients who have been biochemically diagnosed with diabetes mellitus indicates the presence of autoimmune, type 1 diabetes mellitus (aka type 1A diabetes mellitus).
     
  • Prediction of Type 1 Diabetes: The presence of ICA, GADA, IA-2A and/or ZnT8A in the serum of an asymptomatic individual places the subject at increased risk for the development of type 1 diabetes mellitus.
Specimen Requirements: 

Collect blood in a 5 mL serum separator tube. Centrifuge, separate and transfer the serum to a plastic vial. Ship specimens with a frozen cold pack for overnight delivery.

Stability (collection to shipping of the sample):
  • Ambient: 24 hours
  • Refrigerated: 24 hours
  • Frozen: 1 year (Avoid repeated freeze/thaw cycles.)
Reference Values: 
  • ICA:
    • Negative (quantitative results reported in JDF units): < 10 JDF units
    • Positive: ≥ 10 JDF units
       
  • GADA:
    • Negative: < 1.1
    • Positive: ≥ 1.1 U/mL
       
  • IA-2A:
    • Negative: < 0.9
    • Positive: ≥ 0.9
       
  • ZnT8A:
    • Negative: < 15 U/mL
    • Positive: > 15 U/mL
CPT Code (s): 
  • 88346
     
  • 86341 (x3)
Notes: 

UFHPL Test #: 60015

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