Alpha-Fetoprotein (AFP) Tetra Profile

Methodology: 
Immunochemiluminometric assay (ICMA)
Performed: 
Monday - Friday
Use: 
This is a screening test for:
  • Open neural tube defects (detects 80 percent of open spina bifida and 90 percent of anencephaly)
  • Down syndrome (detects 75 to 80 percent)
  • Trisomy 18 (detects 73 percent)

Limitations

A positive AFP tetra result means that more diagnostic testing may be offered to the patient to determine if a neural tube defect or chromosome abnormality is present.

Specimen Requirements: 
Type: Serum
 
Container/Tube: Gel-barrier tube (no thrombin additive)


Sample Volume: 5 mL

Minimum Volume: 3 mL

Storage: Store specimens at room temperature after collection.

Stability (collection to time of analysis/testing): 
  • Ambient: 7 days
  • Refrigerated: 14 days
  • Frozen: 14 days
  • Freeze/Thaw cycles: Stable (x3)
Rejection Criteria:
  • Gross hemolysis
  • Gross lipemia
  • Quantity not sufficient for analysis (QNS samples)
CPT Code (s): 
82105, 82677, 84702, 86336
Notes: 

UFHPL Test #: 28005

UFHPL Epic order code: LAB4509

References

  • Haddow JE, Palomaki GE, Knight GJ, Foster DL, Neveux LM. Second trimester screening for Down's syndrome using maternal serum dimeric inhibin A. J Med Screen. 1998; 5(3):115-119. PubMed 9795869
Collection procedure: 
Avoid hemolysis. Submit the complete specimen in its original tube. Do not pour off.
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