Albumin (ALB)

In 1965, Rodkey introduced a convenient, direct method for determining albumin concentrations in serum utilizing a neutral buffered solution of bromocresol green (BCG) as the dye binding indicator. In 1971, Doumas et al. increased the sensitivity of the reaction by adding a nonionic surfactant to the reagent to prevent turbidity and improve linearity. This Albumin method is a modification of the Doumas and Rodkey procedures utilizing a different buffering system. At pH 4.2, bromocresol green reacts with albumin to form an intense green complex. The absorbance of the albumin-BCG complex is measured bichromatically (600/800nm) and is proportional to the albumin concentration in the sample.
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Purpose and principle: 

Serum albumin measurements are used in the diagnosis of numerous diseases. Elevated serum albumin levels are usually the result of dehydration.

Decreased serum albumin levels are found in a number of conditions, including kidney/liver disease, infections, severe burns and cancer.

Specimen Requirements: 

Type: Peripheral blood

  • Serum, gel
  • Plasma, gel

Sample Volume: 0.5mL serum; sshueparate serum or plasma from cells within two hours of collection.

Stability (collection to time of analysis/testing):
  • Ambient: 7 days
  • Refrigerated: 30 days
Unacceptable Conditions: Moderate-to-gross hemolysis
Reference Values: 
3.5 - 5.7 g/dL
CPT Code (s): 

UFHPL Test #: 20015

UFHPL Epic order code: LAB45

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