Anti-Müllerian Hormone (AMH)

Methodology: 
Electrochemiluminescence (ECL)
Performed: 
Monday - Friday
Specimen Requirements: 
Important: Specimens should be free of bacterial contamination, hemolysis and lipemia.
 

Type: Serum, frozen

Container/Tube: Red-top tube or gel-barrier tube
  • Transfer specimens to a plastic transport tube before freezing. To avoid delays in turnaround time when requesting multiple tests on frozen samples, submit separate frozen specimens for each test requested.

Sample Volume: 1 mL

Minimum Volume: 0.2 mL (Repeat testing is not possible with this specimen volume.)

Storage: Store specimens at room temperature after collection.

Stability (collection to time of analysis/testing): 
  • Ambient: 8 hours
  • Refrigerated: 14 days
  • Frozen: 14 days
  • Freeze/Thaw cycles: Stable (x6)
Rejection Criteria:
  • Gross hemolysis
  • Gross lipemia
  • Unstable specimen
Reference Values: 

Reference Intervals

Age

Range

Median

Male

Birth to 6 years old

32.77 − 262.69

124.58

7 − 10 years old

45.26 − 191.34

92.52

20 − 98years old

0.11 − 13.07

4.95

Female

0 − 6 years old

0.53 − 7.78

2.85

7 − 19 years old

1.05 − 12.86

5.23

20 − 25 years old

1.23 − 11.51

4.70

26 − 30 years old

1.03 − 11.10

4.20

31 − 35 years old

0.66 − 8.75

3.00

36 − 40 years old

0.42 − 8.34

1.69

41 − 46 years old

0.26 − 5.81

0.58

47 − 54 years old

≤ 0.82

< 0.03

55 − 97 years old

≤ 0.18

< 0.03

CPT Code (s): 
82397
Notes: 

UFHPL Test #: 28020

UFHPL Epic order code: LAB4752

References

  • American College of Obstetricians and Gynecologists. Committee on Gynecologic Practice. Committee Opinion N° 618: Ovarian reserve testing. Obstet Gynecol. 2015 Jan; 125(1):268-273. PubMed 25560143
  • Broer SL, Broekmans FJ, Laven JS, Fauser BC. Anti-Müllerian hormone: Ovarian reserve testing and its potential clinical implications. Hum Reprod Update. 2014 Sep-Oct; 20(5):688-701. PubMed 24821925
  • Fleming R, Broekmans F, Calhaz-Jorge C, et. al. Can anti-Müllerian hormone concentrations be used to determine gonadotrophin dose and treatment protocol for ovarian stimulation? Reprod Biomed Online. 2013 May; 26(5):431-439. PubMed 23507133
  • Gleicher N, Weghofer A, Barad DH. Anti-Müllerian hormone (AMH) defines, independent of age, low versus good live-birth chances in women with severely diminished ovarian reserve. Fertil Steril. 2010 Dec; 94(7): 2824-2827. PubMed 20538269
  • Karkanaki A, Vosnakis C, Panidis D. The clinical significance of anti-Mullerian hormone evaluation in gynecological endocrinology. Hormones (Athens). 2011 Apr-Jun; 10(2): 95-103. PubMed 21724534
  • La Marca A, Broekmans FJ, Volpe A, Fauser BC, Macklon NS; ESHRE Special Interest Group for Reproductive Endocrinology—AMH Round Table. Anti-Müllerian hormone (AMH): What do we still need to know? Hum Reprod. 2009 Sep; 24(9):2264-2275. PubMed 19520713
  • La Marca A, Sighinolfi G, Radi D, et al. Anti-Müllerian hormone (AMH) as a predictive marker in assisted reproductive technology (ART). Hum Reprod Update. 2010 Mar-Apr; 16(2):113-130. PubMed 19793843
  • Ledger WL. Clinical utility of measurement of anti-Müllerian hormone in reproductive endocrinology. J Clin Endocrinol Metab. 2010 Dec; 95(12):5144-5154. PubMed 21131535
  • van Disseldorp J, Faddy MJ, Themmen APN, et al. Relationship of serum antimüllerian hormone concentration to age at menopause. J Clin Endocrinol Metab. 2008 Jun; 93(6):2129-2134. PubMed 18334591
  • van Rooij IA, Broekmans FJ, Scheffer GJ, et al. Serum antimüllerian hormone levels best reflect the reproductive decline with age in normal women with proven fertility: A longitudinal study. Fertil Steril. 2005; 83(4):979-987. PubMed 15820810
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