Amino Acids

Methodology: 
Liquid chromatography - tandem mass spectrometry (LC-MS/MS) after derivation by benzoyl chloride
Performed: 
Monday - Friday; STAT is available after consultation with the Metabolics Laboratory director; call 352.265.9900 to make arrangements for a STAT case.
Reported: 
Within 5 business days
Use: 
This test is used to evaluate and monitor amino acidemias, as well as monitor patients on amino acid- and protein-restricted diets.

Reports include data on amino acids used for the diagnosis and monitoring of inherited metabolic disorders.
Specimen Requirements: 
Type: Blood
 
Container: Heparin is preferred, but EDTA is acceptable.
 
Sample volume: 0.5 mL
 
Minimum volume: 0.25 mL
 
Storage: Refrigerate whole blood specimens.
 
Stability (collection to time of analysis):
  • Ambient: 2 hours
  • Refrigerated: 1 day
  • Frozen: 4 weeks
Rejection criteria:
  • Frozen whole blood
  • Citrate or acid citrate dextrose (ACD) tubes
CPT Code (s): 
82139
Notes: 

UFHPL Test #: 40015

Collection procedure: 
Special instructions:
  1. Venipuncture is preferred. Capillary specimens are acceptable, but results for several amino acids *serine, glycine, glutamine, glutamic acid) may not reflect plasma amino acid concentrations.
     
  2. Spin specimens before refrigerating them, if blood is collected in a gel tube (PST, PPT).
     
  3. If transport is delayed or specimens are sent by overnight courier, spin blood and seperate and freeze plasma.
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