Amylase

Methodology: 
This Amylase procedure utilizes 2-chloro-4-nitrophenyl-α-D-maltotrioside (CNPG3) as substrate.2 This substrate reacts directly with α-Amylase and does not require the presence of ancillary enzymes. The release of 2-chloro-4-nitrophenol (CNP) from the substrate and the resulting absorbance increase per minute is directly related to the α-Amylase activity in the sample. The resulting increase in absorbance can be measured spectrophotometrically at 410/480nm. (Package Insert: OSR General Chemistry BAOSR6x06.02; 2012-01)
Performed: 
Monday - Friday
Reported: 
Within 1 day
Use: 
Amylase is found primarily in the pancreas and salivary glands. Elevated serum levels are associated with acute pancreatitis and other pancreatic disorders as well as mumps and bacterial parotitis.
Specimen Requirements: 

Type: Serum

Container/Tube: Serum gel

Sample volume: Submit full tube

Minimum volume:  0.5 mL serum

Preparation:
  1. Send specimen in original tube.
  2.  If there is a delay in transport of more than 1 hour, centrifuge the tube.
Stability (collection to shipping of the sample):
  • Ambient (15 - 25°C): 7 days
  • Refrigerated (2 – 8ºC): 30 days

Unacceptable Conditions: Samples with moderate or marked hemolysis will be rejected.

Specimen Retention Time: 5 days

Reference Values: 

Serum:  29 - 103 U/L

CPT Code (s): 

82150

Notes: 

UFHPL Test #:

UFHPL Epic order code: LAB48

Test Directory