Antibody Screen

Methodology: 
Antiglobulin test
Performed: 
Monday - Friday
Use: 
This test is used to detect atypical IgG antibodies prior to transfusion or during pregnancy. The technique is designed specifically to detect IgG antibodies, though on occasion, some IgM antibodies may also be detected. Antibodies detected by the antibody screen will be subsequently identified, and a titer performed if the antibody identified is considered to be clinically significant during pregnancy.
 

Limitations

No antibody screening test will detect all antibodies. This method may not detect IgM antibodies or antibodies to low frequency antigens. Extremely low-titered antibodies may be detected but may be too weak to identify.
 

Contraindications

Specimens known to test positive for red cell antibody screening should not be submitted for antibody screen testing. In order to facilitate proper routing and testing, Antibody Identification (006213) should be ordered. This test should not be ordered for the husband or partner of a prenatal patient since antibodies detected in the serum or plasma of these individuals are irrelevant during pregnancy.
Specimen Requirements: 
Type: Whole blood
  • This should not be a shared specimen. If antibody identification is necessary, the entire volume of this lavender-top tube will be required.
Container/Tube: Lavender-top tube (EDTA)
 

Sample Volume: 4 mL

Minimum Volume: 1 mL (Repeat testing is not possible with this specimen volume.)

Storage: Specimens should be tested immediately or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2° - 8°C. Do not freeze red cells.

Rejection Criteria:
  • Gross hemolysis
  • Bacterial or other contamination
Reference Values: 
Negative
CPT Code (s): 
86850
Notes: 

UFHPL Test #: 68025

UFHPL Epic order code: LAB278

References

  • Fung MK, Grossman JB, Hillyer CD, Westhoff CM, eds. AABB Technical Manual. 18th ed. 2014.
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