Antinuclear Antibodies (ANA) Comprehensive Profile

Methodology: 
Multiplex flow immunoassay
Performed: 
Monday - Friday
Use: 
This test is used to detect antibodies associated with SLE and mixed connective tissue disease. Anti-Sm is highly specific for SLE. Anti-RNP is found with a variety of rheumatoid diseases. RNP, Sm, SS-A, SS-B, Scl-70, and Jo-1 are extractable nucleoprotein complexes found in the nucleus and/or cytoplasm of substrate cells.
 
The test includes:
  • Anticentromere B antibodies
  • Anti-dsDNA
  • Antichromatin antibodies
  • Anti-Jo-1; RNP antibodies
  • Antiscleroderma 70 antibodies
  • Smith antibodies
  • Sjögren anti-SS-A
  • Sjögren anti-SS-B
Specimen Requirements: 

Type: Serum 

Container/Tube: Red-top tube or gel-barrier tube
 

Sample Volume: 1 mL

Minimum Volume: 0.5 mL (Repeat testing is not possible with this specimen volume.)

Storage: Store specimens at room temperature.

Stability (collection to time of analysis/testing):
  • Ambient: 14 days
  • Refrigerated: 14 days
  • Frozen: 14 days
  • Freeze/Thaw Cycles: Stable (x 3)
Rejection Criteria:
  • Hemolysis
  • Lipemia
  • Gross bacterial contamination
  • Icterus
CPT Code (s): 
86225, 86235 (x 8)
Notes: 

UFHPL Test #: 68015

UFHPL Epic order code: LAB210768

References

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