Antistreptolysin O (ASO) Antibodies

Methodology: 
Latex immunoturbidimetry
Performed: 
Monday - Friday
Use: 
This test is used to document exposure to streptococcal streptolysin O. A marked rise in titer or a persistently elevated titer indicates that a Streptococcus infection or poststreptococcal sequelae are present.
 

Limitations

False-positive ASO titers can be caused by increased levels of serum β-lipoprotein produced in liver disease and by contamination of the serum with Bacillus cereus and Pseudomonas. ASO titers are elevated in 85 percent of patients with rheumatic fever but may not be elevated in cases involving skin or renal sequelae.
Specimen Requirements: 

Type: Serum 

Container/Tube: Red-top tube or gel-barrier tube
  • If a red-top tube is used, transfer the separated serum to a plastic transport tube.

Sample Volume: 2 mL

Storage: Store specimens at room temperature.

Stability (collection to time of analysis/testing):
  • Ambient: 14 days
  • Refrigerated: 14 days
  • Frozen: 14 days
  • Freeze/Thaw Cycles: Stable (x 3)
Rejection Criteria:
  • Whole blood
  • Plasma other than EDTA
  • Hemolysis
  • Room temperature or refrigerated serum
CPT Code (s): 
86060
Notes: 

UFHPL Test #: 68040

Streptolysin is a hemolysin produced by group A streptococci. In an infected individual streptolysin O acts as a protein antigen, and the patient mounts an antibody response. A rise in antibody level begins about one week after infection and peaks two to three weeks later. In the absence of complications or reinfection, the ASO titer will usually fall to preinfection levels within 6 to 12 months. Both clinical and laboratory findings should be correlated in reaching a diagnosis.
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