Bicarbonate (CO2)

Methodology: 
The bicarbonate reagent utilizes the enzymatic method developed by Forrester et al. In this procedure bicarbonate (HCO3¯) and phosphoenolpyruvate (PEP) are converted to oxaloacetate and phosphate in the reaction catalyzed by phosphoenolpyruvate carboxylase (PEPC). Malate dehydrogenase (MD) catalyzes the reduction of oxaloacetate to malate with the concomitant oxidation of reduced nicotinamide adenine dinucleotide (NADH). This oxidation of NADH results in a decrease in absorbance of the reaction mixture measured bichromatically at 380/410 nm proportional to the Bicarbonate content of the sample.
Performed: 
Monday - Friday
Reported: 
Within 24 hours
Reported Notes: 
Specimen Retention Time: 5 days
Purpose and principle: 

Bicarbonate measurements are used in the diagnosis and treatment of numerous potentially serious disorders associated with changes in body acid-base balance. The determination of bicarbonate (HCO3¯) is used in conjunction with other clinical and laboratory information for the evaluation of acid-base status. An elevation of the bicarbonate level may be observed in compensated respiratory acidosis and metabolic alkalosis. Low bicarbonate levels may be observed in compensated respiratory alkalosis and metabolic acidosis.1 Additional laboratory determinations will permit differentiation between metabolic and respiratory conditions.

Specimen Requirements: 
Type: Peripheral blood
 
Container/Tube:
  • Serum, gel
  • Plasma, gel

Sample Volume: 0.5mL

Stability (collection to time of analysis/testing): Protect from exposure to air.
  • Ambient: 24 hours
Unacceptable Conditions: Moderate to gross hemolysis
Notes: 

UFHPL Test #: 20088

Collection procedure: 
Serum gel tubes should be centrifuged within 2 hours of collection.
Test Directory