CD4:CD8 Ratio Profile

Methodology: 
Flow cytometry
Performed: 
Monday - Friday
Use: 
This test includes:
  • Percentage CD4+
  • Absolute CD4+
  • Percentage CD8+
  • Absolute CD8+
  • CD4:CD8 ratio
  • CBC with differential
Specimen Requirements: 

Type: Whole blood

Container/Tube: Lavender-top tube (EDTA) and yellow-top tube (ACD-A) or (ACD-B)
  • Invert each tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimens so they will arrive at the laboratory within 48 hours of collection. Indicate the date and time of venipuncture on the tubes and on the test requisition.

Sample Volume: Fill the tubes to capacity.

Storage: Store specimens at room temperaturen.

Stability (collection to time of analysis/testing):
  • Ambient: 2 days
  • Refrigerated: Unstable
  • Frozen: Unstable
  • Freeze/Thaw Cycles: Unstable
Rejection Criteria:
  • Hemolysis
  • Specimen refrigerated or frozen
  • Clotted specimen
  • Contaminated specimen
Reference Values: 

Reference Intervals

CD

Age

95 Percent Confidence Interval

Percentage

Cells/mm3

Minimum

Maximum

Minimum

Maximum

Pediatric reference intervals are from Comans-Bitter WM, de Groot R, van den Beemd R, et al. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatrics. 1997 Mar; 130(3):388-393.

CD4+

Neonates

17

52

400

3500

1 week to
2 months old

41

68

1700

5300

2 − 5
months old

33

58

1500

5000

5 − 9
months old

33

58

1400

5100

9 − 15
months old

31

54

1000

4600

15 − 24
months old

25

50

900

5500

2 − 5
years old

23

48

500

2400

5 − 10
years old

27

53

300

2000

10 − 16
years old

25

48

400

2100

Adults

31

59

359

1519

CD8+

Neonates

10

41

200

1900

1 week to
2 months old

9

23

400

1700

2 − 5
months old

11

25

500

1600

5 − 9
months old

13

26

600

2200

9 − 15
months old

12

28

400

2100

15 − 24
months old

11

32

400

2300

2 − 5
years old

14

33

300

1600

5 − 10
years old

19

34

300

1800

10 − 16
years old

9

35

200

1200

Adults

12

36

109

897

T4:T8 ratio

Adults

0.92

3.72

   
CPT Code (s): 
86360
Notes: 

UFHPL Test #: 68055

UFHPL Epic order code: LAB4477

HIV-1 infection results in a decrease of CD4 T cells, an increase of CD8 T cells, a decrease in the CD4:CD8 ratio, and a progressive destruction of immune function. In HIV-1 seropositive patients, enumeration of CD4 T cells may be used for prognostic purposes and to monitor disease progression and antiretroviral therapy.
 

References

  • Centers for Disease Control and Prevention (CDC). 1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV). MMWR. 1997 Jan 10; 46:RR-2.
  • Giorgi J, Kesson A, Chou CC. Immunodeficiency and infectious diseases. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 25.
  • Stevens RA, Lempicki RA, Natarajan V, et al. General immunologic evaluation of patients with immunodeficiency virus infection. In: Detrick B, Hamilton RG, Folds JD, et al, eds. Manual of Molecular and Clinical Laboratory Immunology. 7th ed. Washington, DC: ASM Press;2006:chap 95.
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