Chromosome Study: Neoplastic Lymphatic Tissue

Methodology: 
Classic cytogenetic G-band analysis (karyotyping); requires cell culture
Performed: 
Monday - Saturday
Reported: 
7 - 21 days
Use: 

This test is used to identify clonal chromosome changes by conventional karyotyping methods associated with various lymphoma subtypes.

Specimen Requirements: 

Lymph Node/Spleen/Other: Aseptically obtain a sample of approximately 2 mm x 2 mm x 2 mm and deposit it in RPMI tissue culture medium.*

Fine-Needle Aspirates: Deliver the contents in RPMI or sterile tissue culture medium in a 15 mL conical tube.*

Unacceptable Conditions:
  • Frozen specimens
  • Specimens greater than 48 hours old*
Stability (collection to initiation of testing):
  • Ambient: 24 - 48 hours old*
  • Frozen: Unacceptable

* Cell viability of specimens requiring cell culture may be compromised in specimens greater than 48 hours old upon receipt. Refrigeration at 2° - 8° C may assist when a delay in delivery is unpreventable. Do not freeze or place specimens directly on ice.

No formalin-, alcohol- or paraffin-preserved specimens. Do not freeze or fix samples in any manner contrary to the maintenance of cell viability. Maintain sample sterility; keep all specimens at room temperature; and deliver samples to the laboratory within 24 - 48 hours. Ship specimens at room temperature for overnight delivery.

Reference Values: 

By report; interpretive

CPT Code (s): 
  • 88239
  • 88262
  • 88291
Notes: 

UFHPL Test #: 30035

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