Epidermal Growth Factor Receptor Mutation Analysis (EGFR Mutation)

Methodology: 
Real-time polymerase reaction
Performed: 
Weekly
Reported: 
Within 10 business days
Use: 

This test is used to detect EGFR somatic mutations in exons 18, 19, 20 and 21. EGFR mutation is significantly associated with response to gefitinib and erlotinib and prolonged survival in non-small-cell lung carcinoma patients.

Specimen Requirements: 

At least 7 unstained formalin-fixed paraffin-embedded slides with adequate amounts of tumor to be analyzed with the areas of tumor marked.

Storage/Transport: Ship specimens at 20° - 25° C for overnight delivery and protect them from excessive heat.  Include a surgical pathology report.

Unacceptable Specimens:
  • Specimens that do not contain tumor
  • Specimens in fixatives other than 10% formalin
  • Frozen specimens
Stability (collection to initiation of testing):
  • Ambient: Indefinitely
  • Refrigerated: Acceptable
  • Frozen: Unacceptable
CPT Code (s): 
  • 81235 (needs confirmation)
  • 88381 (FFPE)
Notes: 

UFHPL Test #: 10075

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