Estrogens, Total

Methodology: 
Radioimmunoassay (RIA)
Use: 
This test is used to evaluate ovarian estrogen producing tumor in the pre-menarcheal and postmenopausal females, as well as estrogen excess in males. Estrogen analysis may be helpful in establishing time of ovulation and optimal time for conception. Serial samples must be collected over several days to evaluate baseline and peak total estrogen levels.
Specimen Requirements: 
Type: Serum or plasma
 
Container/Tube: Red-top tube, gel-barrier tube or green-top tube
  • If a tube other than a gel-barrier tube is used, transfer the separated serum or plasma to a plastic transport tube.

Sample Volume: 2.8 mL

Minimum Volume: 2.2 mL (Repeat testing is not possible with this specimen volume.)

Storage: Refrigerate specimens after collection.

Stability (collection to time of analysis/testing): 
  • Ambient: 3 days
  • Refrigerated: 7 days
  • Frozen: 14 days
  • Freeze/Thaw cycles: Stable (x3)
Rejection Criteria:
  • Gross hemolysis
  • A recent isotopic scan
  • Gross lipemia
  • Icteric specimen
Reference Values: 

Reference Intervals

Age

Male
(pg/mL)

Female
(pg/mL)

Pre-pubertal

< 40

< 40

Adults

40 - 115

Female Cycle

Castrate: < 40

1 - 10 days: 61 - 394

11 - 20 days: 122 - 437

21 - 30 days: 156 - 350

Postmenopausal: < 40

HMG treatment for
ovulation induction:
400 - 800

Tanner Stage

I

10 - 38

10 - 46

II

17 - 45

22 - 63

III

22 - 55

24 - 110

IV

27 - 80

40 - 180

V

25 - 80

60 - 280

CPT Code (s): 

82672

Notes: 

UFHPL Test #: 28075

UFHPL Epic order code: LAB981

Footnotes

  1. Tietz NW, ed. Clinical Guide to Laboratory Tests. 3rd ed. Philadelphia, Pa: WB Saunders Co;1995:220.

References

  • Ashby CD, Danzer HC, Swerdloff RS, et al. Estrogen radioimmunoassay suitable for the monitoring of ovulation induction. Clin Chem. 1980; 26(8):1143-1146. PubMed 7389084
  • Marshall JR. Induction of ovulation. Clin Obstet Gynecol. 1978; 21(1):147-162 (review). PubMed 343954
  • Taymor ML, Yussman MA, Gminski D, et al. Estrogen monitoring in ovulation induction. Fertil Steril. 1970; 21(11):759-762.PubMed 5474219
Collection procedure: 
Patients must avoid having a radioisotope scan prior to collection of  the specimen.
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