Estrone, Serum

Methodology: 
Radioimmunoassay (RIA)
Performed: 
Monday - Friday
Use: 
This test is used to evaluate postmenopausal vaginal bleeding due to peripheral conversion of androgenic steroids. Increased estrone levels may be associated with increased levels of circulating androgens and their subsequent peripheral conversion.
Specimen Requirements: 
Type: Serum

Note: No isotopes should be administered to the patient within 24 hours prior to the venipuncture.
 
Container/Tube: Red-top tube, gel-barrier tube or lavender-top tube (EDTA)
  • If a red-top tube or lavender-top tube is used, transfer the separated serum to a plastic transport tube.

Sample Volume: 0.8 mL

Minimum Volume: 0.4 mL (Repeat testing is not possible with this specimen volume.)

Storage: Refrigerate specimens after collection.

Stability (collection to time of analysis/testing): 
  • Ambient: 3 days
  • Refrigerated: 7 days
  • Frozen: 14 days
  • Freeze/Thaw cycles: Stable (x3)
Rejection Criteria:
  • Gross hemolysis
  • Recently administered radioisotopes
  • Lipemia
  • Icteric specimen
Reference Values: 

Reference Intervals

Age
(in years)

 

Male
(pg/mL)

 

Female
(pg/mL)

0 - 5

18 - 53

19 - 46

6 - 7

17 - 48

17 - 44

8 - 9

20 - 54

31 - 70

10 - 11

21 - 49

28 - 68

12 - 14

17 - 44

57 - 140

Adults

12 - 72

 

Follicular Phase: 37 - 138
Mid-cycle: 60 - 229
Luteal Phase: 50 - 114
Postmenopausal: 14 - 103


Tanner Stage
 

I

5 - 17

4 - 29

II

10 - 25

10 - 33

III

15 - 25

15 - 43

IV

15 - 45

16 - 77

V

20 - 45

29 - 105

CPT Code (s): 
82679
Notes: 

UFHPL Test #: 28080

UFHPL Epic order code: LAB982

Footnotes

  1. Meites S, Buffone GJ, Cheng MH, et al, eds. Pediatric Clinical Chemistry, Reference (Normal) Values. 3rd ed. Washington, DC: AACC Press; 1989:123.
  2. Ducharme JR, Forest MG, De Peretti E, et al. Plasma adrenal and gonadal sex steroids in human pubertal development. J Clin Endocrinol Metab. 1976; 42(3):468-476. PubMed 130382
  3. Tietz NW, ed. Clinical Guide to Laboratory Tests. 3rd ed. Philadelphia, Pa: WB Saunders Co;1995:222.

References

  • Baird DT, Fraser IS. Concentration of oestrone and oestradiol in follicular fluid and ovarian venous blood in women. Clin Endocrinol (Oxf). 1975; 4(3):259-266. PubMed 1149301
  • Wright K, Robinson H, Collins DC, Preedy JR. Investigation of a radioimmunoassay for plasma estrone and estradiol-17-β in male and nonpregnant females. Comparison with an independent method using fluorometry. J Clin Endocrinol Metab. 1973 Jan; 36(1):165-173. PubMed 4734134
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