FISH Study: Neoplastic Interphase ATM (deletion 11q22.3)

Methodology: 
Interphase nuclei fluorescence in-situ hybridization (FISH) fusion rearrangement analyses
Performed: 
Monday - Saturday
Reported: 
5 - 12 days (subject to a broad range of variables)
Use: 

This test is used to detect deletions of the long-arm of chromosome 11, including the ATM gene locus, which is commonly associated with CLL/SLL.

Result: 
Interpretive report
Specimen Requirements: 
  • Bone Marrow Aspirate: Obtain 1 - 2 cc of in a green-top sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
  • Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) which has been brought to room temperature prior to collection.*
  • Peripheral Blood Specimens: Peripheral blood specimens may be submitted in those clinical conditions (i.e. circulating blasts) and/or circumstances (where warranted).

Do not freeze or fix samples in any manner contrary to the maintenance of cell viability. Maintain sample sterility; keep all specimens at room temperature; and deliver samples to the laboratory within 24 - 48 hours. Ship specimens at room temperature for overnight delivery.

  • Unacceptable Conditions:
    • Frozen specimens
    • Specimens for FISH study only greater than one week old
    • Specimens for concurrent FISH and cytogenetic studies greater than 48 hours old*
    • Formalin, alcohol or paraffin-preserved specimens
  • Stability (collection to initiation of testing):
    • Ambient: 24 - 48 hours*
    • Frozen: Unacceptable

* Cell viability of specimens requiring cell culture may be compromised in specimens more than 48 hours old upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.

Reference Values: 

By report; interpretive

CPT Code (s): 
  • 88271
  • 88275
  • 88291
Notes: 

UFHPL Test #: 30090

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