Flow Cytometry on Peripheral Blood
This test is used to diagnose hematolymphoid malignancies in blood.
One 5 - 10 mL specimen of peripheral blood in a lavender- (EDTA), green- (sodium heparin) or yellow-top (ACD) tube is accepted.
If cytogenetics studies are requested, one additional green-top sodium heparin tube with 5 mL of peripheral blood specimen should be submitted.
A freshly prepared peripheral blood smear is also beneficial.
Storage/Transport Temperature: The fresh tissue or fluid specimen can be transported with a cold pack or wet ice, but do not fix or freeze specimens (no dry ice).
- Frozen specimens
- Specimens greater than 24 hours old
- Inaccurately labeled specimens
- Ambient: 12 hours
- Refrigerated: 24 hours
Other Requirements: A copy of the CBC and differential with clinical history should be submitted with the specimens. For your convenience, you may also check the boxes on the requisition form to provide brief clinical information. Although we accept specimens greater than 24 hours old after collection, the viability of cells may deteriorate over time. Clotted, hemolyzed and frozen samples are unacceptable.
For more information, contact the UF Health Pathology Laboratories Client Services Department at 888.375.LABS (5227).
- 85060 Peripheral blood review
- 88108 Cytospin technical
- 88184 First marker flow cytometry, technical
- 88185 Each additional marker, flow cytometry technical
- 88187 Flow cytometry interpretation, 2 - 8 markers
- 88188 Flow cytometry interpretation, 9 - 15 markers
- 88189 Flow cytometry interpretation, 16 or more markers
- 88319 Myeloperoxidase or non-specific esterase stain (for acute leukemia)