Flow Cytometry on Peripheral Blood

Methodology: 
Flow cytometry with or without microscopic examination
Performed: 
Monday - Saturday
Reported: 
Within 2 days (Stat cases may be completed within 24 hours.)
Use: 

This test is used to diagnose hematolymphoid malignancies in blood.

Specimen Requirements: 

One 5 - 10 mL specimen of peripheral blood in a lavender- (EDTA), green- (sodium heparin) or yellow-top (ACD) tube is accepted.

If cytogenetics studies are requested, one additional green-top sodium heparin tube with 5 mL of peripheral blood specimen should be submitted.

A freshly prepared peripheral blood smear is also beneficial.

Storage/Transport Temperature: The fresh tissue or fluid specimen can be transported with a cold pack or wet ice, but do not fix or freeze specimens (no dry ice).

Unacceptable Conditions:
  • Frozen specimens
  • Specimens greater than 24 hours old
  • Inaccurately labeled specimens
Stability (collection to initiation of testing):
  • Ambient: 12 hours
  • Refrigerated: 24 hours

Other Requirements: A copy of the CBC and differential with clinical history should be submitted with the specimens. For your convenience, you may also check the boxes on the requisition form to provide brief clinical information. Although we accept specimens greater than 24 hours old after collection, the viability of cells may deteriorate over time. Clotted, hemolyzed and frozen samples are unacceptable.

For more information, contact the UF Health Pathology Laboratories Client Services Department at 888.375.LABS (5227).

Reference Values: 

Interpretive report

CPT Code (s): 
CPT coding varies, depending upon actual testing performed, but common CPT codes are listed as the following:
  • 85060 Peripheral blood review
  • 88108 Cytospin technical
  • 88184 First marker flow cytometry, technical
  • 88185 Each additional marker, flow cytometry technical
  • 88187 Flow cytometry interpretation, 2 - 8 markers
  • 88188 Flow cytometry interpretation, 9 - 15 markers
  • 88189 Flow cytometry interpretation, 16 or more markers
  • 88319 Myeloperoxidase or non-specific esterase stain (for acute leukemia)
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