GatorSeq (NGS)

Performed: 
Monday - Friday
Reported: 
10 - 14 days
Use: 
This test is used to determine a prognosis and/or treatment options for individuals with cancer at initial diagnosis or with refractory disease.
Specimen Requirements: 
The preferred specimen is formalin-fixed, paraffin-embedded tissue block; however, fresh tissue or cell suspensions are also acceptable.

Shipping for Fresh/Fresh-Frozen Tissue: Flash-freeze and ship frozen specimens on dry ice for overnight arrival Monday through Friday.

Unacceptable Conditions:
  • Less than 10 percent tumor
  • Specimens fixed/processed in alternative fixatives (alcohol, Anatech Prefer fixative) or heavy metal fixatives
  • Decalcified specimens
Stability (collection to initiation of testing):
  • Frozen (on Dry Ice): 24 - 48 hours  
Interpretation Data: 
No mutation detected; interpretative report provided
CPT Code (s): 
81455
Notes: 

UFHPL Test #: 10145

GatorSeq is a hybridization capture-based next-generation-targeted resequencing assay for deep sequencing of 1207 exons of 76 key cancer genes, encompassing 240,998bp of genomic DNA. Tumor samples are macrodissectedto increase tumor cell percentage in the analyzed sample and sequenced on an Illumina NextSeq to high-uniform depth (targeting 500x coverage by non-polymerase chain reaction- (PCR) duplicate read pairs, with more than 99 percent of exons at coverage greater than 100 times). Sequence data are processed using a customized analysis pipeline that is designed to accurately detect base substitutions and insertions/deletions. Annotated reports are generated using a GenomeOncology-developed software and database.

The entire coding sequences of the following genes are covered by this assay:

  • ABL1
  • AKT1
  • ALK
  • ASXL1
  • BAALC
  • BCOR
  • BCR
  • BRAF
  • CBFB
  • CEBPA
  • CRLF2
  • CTNNB1
  • DDR2
  • DEK
  • DNMT3A
  • EGFR
  • ERBB2
  • ERG
  • ETV6
  • MECOM
  • EZH2
  • BRINP3
  • FGFR1
  • FGFR2
  • FLT3
  • FBXW7
  • GNA11
  • GNAQ
  • HOXA9
  • HRAS
  • IDH1
  • MYC
  • IDH2
  • JAK2
  • KIT
  • KRAS
  • MAP2K1
  • MET
  • MKL1
  • KMT2A
  • MLLT3
  • MN1
  • MPL
  • MYH11
  • NF1
  • NOTCH1
  • NPM1
  • NRAS
  • NUP214
  • PDGFRA
  • PHF6
     
  • PIK3CA
  • PML
  • PTEN
  • PTPN11
  • RAD21
  • RARA
  • RBM15
  • RET
  • RUNX1T1
  • RPN1
  • RUNX1
  • SF3B1
  • SMAD4
  • SMC1A
  • SMC3
  • SMO
  • SRSF2
  • STAG2
  • TET2*
  • TP53
  • TSC1
  • U2AF1
  • U2AF65
  • WT1
  • ZRSR2
     
Test Directory