Hepatitis C Virus (HCV) Genotype

Methodology: 
Polymerase chain reaction (PCR) amplification of estracted nucleic acid followed by a direct analysis of the electrochemical eSensor XT-8 detection system
Performed: 
Weekly
Reported: 
Within 9 business days
Use: 
This test is designed to genotype a panel of eight prevalent HCV types/subtypes (1a, 1b, 2a/c, 2b, 3, 4, 5 and 6), using multiplex reverse transcription (RT).
Specimen Requirements: 
Acceptable Specimens
  • 500uL of serum or plasma (Separate from cells within six hours.)

Storage/Transport Temperature
  • Refrigerated

Unacceptable Conditions
  • Heparinized specimens

Remarks: Submit the most recent viral load and test date along with the specimen (if available).



Stability
  • On Cells:
    • Ambient: Six hours
  • After Separation from Cells:
    • Refrigerated: 72 hours
    • Frozen: Four months

Additional Information: Specimens should contain a recommended minimum hepatitis C viral load of 1000 IU/mL.

CPT Code (s): 
87902
Notes: 
Special Instructions
  • This assay should not be used as a screening test for hepatitis C virus (HCV) infection. It should be requested only on specimens obtained from patients confirmed to have HCV RNA levels in serum that are greater than or equal to 1000 IU/mL.
  • An undetected or indeterminate HCV genotype result does not rule-out active HCV infection. Test results should be correlated with routine serologic and molecular-based testing, as well as clinical presentation.
  • Specimens containing low HCV viral load (i.e. less than 1,000 IU/mL) may yield indeterminate results.

UFHPL Test #: 10090

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