Hemoglobin A1C

In a pretreatment step, whole blood is mixed with the Hemolyzing reagent in a 1:100 dilution and the resultant hemolyate is used. The concentration of both HbA1c and total hemoglobin are determined. The HbA1c/Total Hemoglobin ratio is expressed as %HbA1c. Total hemoglobin reagent is used to measure total hemoglobin concentration by a colorimetric method. Change in absorbance is measure at 570/660 nm. HbA1c reagent is used to measure hemoglobin A1c concentration by a turbidimetric immunoinhibition method. In the reaction, hemoglobin A1c antibodies combine with HbA1c from the sample to form soluble antigen-antibody complexes. Polyhaptens from the reagent then bind with the excess antibodies and the resulting agglutinated complex is measured turbidimetrically. Change in absorbance is measured at 340/700 nm.
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Purpose and principle: 
Measurement of hemoglobin A1c is accepted as a method to measure long-term glucose control in patients with diabetes mellitus. Determination of HbA1c provides an important tool for monitoring the efficiency of dietary control and therapy during treatment of diabetes mellitus. The process of conversion from hemoglobin A to hemoglobin A1c depends on the blood glucose concentration. Since the average life of a red blood cell is 120 days, measurement of hemoglobin A1c can reflect the mean daily blood glucose concentration over the preceding two to three months and provides a much better indication of glycemic control than blood or urinary glucose determinations.
Specimen Requirements: 

Type: Peripheral blood

Container/Tube: EDTA whole blood

Sample Volume: 0.5mL

Stability (collection to time of analysis/testing):
  • Ambient: 8 hours
  • Refrigerated: 7 days
  • Frozen: 3 months
Specimen Retention Time: 5 days
Reference Values: 
4.0 – 6.0%
CPT Code (s): 

UFHPL Test #: 20255

UFHPL Epic order code: LAB90

Collection procedure: 
Serum gel tubes should be centrifuged within 2 hours of collection.
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