Human Immunodeficiency Virus 1/O/2 (HIV-1/O/2) Antibodies, Preliminary Test With Confirmation

Immunochemiluminometric assay (ICMA); reflex to supplemental differentiation assay
Monday - Friday
Specimen Requirements: 

Type: Serum (preferred) or EDTA plasma

Container/Tube: Red-top tube, gel-barrier tube or lavender-top tube (EDTA) (if unopened/unpunctured)
  • No pour-offs. Do not perform additional testing on original tube prior to shipment.

Sample Volume: 4 mL

Storage: Store specimens at room temperature after collection.

Stability (collection to time of analysis/testing):
  • Ambient: 14 days
  • Refrigerated: 14 days
Reference Values: 
CPT Code (s): 
86703 (If reflex testing is performed, additional CPT code(s)/charges will apply.)

UFHPL Test #: 68135

This test includes HIV antibodies by ICMA with HIV-1/HIV-2 supplemental antibody confirmation. If ICMA results are repeatedly positive, reflex to supplemental differentiation assay will be performed at an additional charge. Human immunodeficiency virus (HIV 1/O/2), the etiologic agent of the acquired immunodeficiency syndrome (AIDS), is a cytopathic retrovirus. This test uses recombinant antigen sources and detects antibodies by specific immune binding and subsequent chemiluminescent reaction (ICMA technology). Sensitivity and specificity of this assay are 100 percent and 99.9 percent, respectively. Sera that are repeatedly reactive in two out of three tests will reflex to HIV-1/HIV-2 supplemental testing. Some individuals may be initially reactive by the preliminary test and negative or indeterminate by HIV-1/HIV-2 supplemental testing. This may be caused by other viral antibodies or autoantibodies that cross-react with the viral antigens, although this is extremely rare.

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