Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-Time Polymerase Chain Reaction (PCR)

Methodology: 
Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test (Version 2.0)
Performed: 
Monday - Friday
Use: 
This test is used to detect and quantitate HIV-1 in plasma.
 

Limitations

  • Assay Range: 20 − 10,000,000 copies/mL
Specimen Requirements: 
Type: Plasma
 
Container/Tube: Lavender-top tube (EDTA) or plasma-preparation tube (PPT)
  • Centrifuge the specemin within 24 hours of collection. Transfer the plasma to a screw-cap polypropylene transport tube. Ship specimens to the lab frozen (preferred). To avoid delays in turnaround time when requesting multiple tests on frozen samples, submit a separate frozen specimen for each test requested.
     

Sample Volume: 3.5 mL

Minimum Volume: 1.1 mL

Storage: Freeze (preferred) or refrigerate specimens immediately after collection.
 
Stability (collection to time of analysis/testing): 
  • Ambient: 24 hours
  • Refrigerated: 6 days
  • Frozen: 6 weeks
 
Rejection Criteria:
  • Incorrect anticoagulant
  • PPT frozen and/or not centrifuged
CPT Code (s): 
87536
Notes: 

UFHPL Test #: 78045

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