Immunofixation, Serum

Methodology: 
Electrophoresis followed by immunodiffusion against monospecific antisera to immunoglobulin and individual heavy and light chain (IFE); turbidimetric quantitation of IgA, IgG, and IgM
Performed: 
Monday - Friday
Use: 
This test detects and identifies monoclonal immunoglobulin gammopathies.

Immunofixation electrophoresis of serum or urine is most often ordered to evaluate a monoclonal globulin detected in a protein electrophoresis or to delineate a possible lymphoproliferative process, particularly myeloma. This procedure will characterize the specific light and heavy chain components of a monoclonal protein.
Specimen Requirements: 
Type: Serum (Sperate serum from red cells.)
 

Container/Tube: Red-top tube or gel-barrier tube

Sample Volume: 2 mL

Minimum Volume: 1 mL

Storage: Store specimens at room temperature.

Stability (collection to time of analysis/testing): 
  • Ambient: 14 days
  • Refrigerated: 14 days
  • Frozen: 14 days
  • Freeze/Thaw cycles: Stable (x3)
Causes for Rejection:
  • Gross hemolysis
  • Gross lipemia
Note: The patient's age and clinical diagnosis must be submitted with the test request.
Reference Values: 
Normal pattern
CPT Code (s): 
82784(x3), 86334
Notes: 

UFHPL Test #: 28105

UFHPL Epic order code: LAB4493

Immunofixation electrophoresis of serum or urine is most often ordered to evaluate a monoclonal globulin detected in a protein electrophoresis or to delineate a possible lymphoproliferative process, particularly myeloma. This procedure will characterize the specific light and heavy chain components of a monoclonal protein.
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