Additional Information:
UFHPL Epic order code: LAB96
CPT Code(s):
83615
Collection Procedure:
Serum gel tubes should be centrifuged within 2 hours of collection.
Specimen Requirements:
Type: Peripheral blood
Container/Tube:
- Serum, gel
- Plasma, gel
Sample Volume: 0.5mL
Stability (collection to time of analysis/testing):
- Ambient: ASAP
- Refrigerated: Not recommended
- Frozen: Not recommended
Unacceptable Conditions: Moderate or gross hemolysis
Methodology:
The LD procedure employs a modification of the method of Wacker et al. which utilizes the forward reaction of lactate to pyruvate. Lactate and NAD are converted to pyruvate and NADH catalyzed by LD. NADH strongly absorbs light at 340 nm, whereas NAD does not. The rate of change of absorbance at 340 nm is directly proportional to the LD activity in the sample.
Reported:
Within 24 hours
Reference Values:
140 – 271 U/L
Performed:
Monday - Friday