Latent Autoimmune Diabetes of Adults (LADA)

Methodology: 
GADA: Radioimmunoassay | ZnT8A: Enzyme-linked immunosorbent assay (ELISA)
Performed: 
Weekly
Reported: 
7 - 10 business days
Use: 

Classic type 1 diabetes (T1DM) is of acute onset and most often occurs during childhood. Children usually have symptoms of diabetes for no more than 2 to 6 weeks. A more slowly progressive form of autoimmune diabetes that presents in adulthood is latent autoimmune diabetes of adulthood (LADA) [1]. LADA is therefore a form of T1DM.

Subjects who present with a “non-insulin dependent diabetes” phenotype that carry HLA-DR3 or DR4 are likely to express islet autoantibody and are more likely to become insulin-requiring compared with DR3/DR4-negative type 2 diabetes (T2DM) subjects [2].

The most sensitive islet autoantibody markers of LADA are glutamic acid decarboxylase autoantibodies (GADA) and zinc transporter 8 autoantibodies (ZnT8A).

References

  1. Brahmkshatriya PP, Mehta AA, Saboo BD, Goyal RK. Characteristics and prevalence of latent autoimmune diabetes in adults (LADA). ISRN Pharmacol 2012;2012:580202.
  2. Kitabchi AE, Umpierrez GE, Murphy MB, Kreisberg RA. Hyperglycemic crises in adult patients with diabetes: a consensus statement from the American Diabetes Association. Diabetes Care. 2006 Dec;29(12):2739-48.
Specimen Requirements: 

Collect blood in a 1 - 5 mL serum separator tube. Centrifuge, separate and transfer the serum to a plastic vial. Ship specimens with a frozen cold pack for overnight delivery.

Stability (collection to shipping of the sample):

  • Ambient: 24 hours
  • Refrigerated: 24 hours
  • Frozen: 1 year (Avoid repeated freeze/thaw cycles.)
Reference Values: 
  • GADA:
    • Negative: < 1.0 U/mL
    • Positive: > 1.0 U/mL
  • ZnT8A
    • Negative : < 15  U/mL
    • Positive: > 15  U/mL
CPT Code (s): 
86341 (x 2)
Notes: 

UFHPL Test#: 60102

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