Protein, Random Urine

Additional Information:

UFHPL Epic order code: LAB439

CPT Code(s):

84156

Specimen Requirements:

Type: Urine, random

Container/Tube: Plastic urine container with no preservative

Sample Volume: 1 mL

Storage: Refrigerate specimens.

Specimen Retention Time: 5 days

Use:

This test is used in the measurement of total protein in urine, which is important in the diagnosis and treatment of diseases associated with renal, cardiac and thyroid function.

Methodology:

The urinary/CSF protein reagent is a colorimetric method. Pyrogallol red is combined with molybdate to form a red complex with a maximum absorbance at 470nm. The assay is based on the shift in absorbance that occurs when the pyrogallol red-molybdate complex binds basic amino groups of protein molecules. Under the conditions of the test in the presence of protein, a blue-purple complex is formed with a maximum absorbance at 600nm. The absorbance of this complex is directly proportional to the protein concentration in the sample. (Package insert, OSR General Chemistry BAOSR6x70.03, 2013-08)

Reported:

Within 24 hours

Reference Values:

No reference range established

Performed:

Monday - Friday