ABO Grouping and Rho(D) Typing

Additional Information:

EPIC Test Name: ABO/RH TYPE
UFHPL Epic Order Code: LAB895

CPT Code(s):

86900, 86901

Collection Procedure:

Special Instructions: If a CBC and/or an antibody screening test is ordered with this test, submit a separate 4 mL specimen for each test.

Specimen Requirements:

Type: Whole blood

Container/Tube: Lavender (EDTA)

  • Storage. Refrigerate.

Sample Volume: 4 mL

Minimum Volume: 1 mL (Repeat testing is not possible with this specimen volume.)

Use:

Determination of ABO blood group and Rh type for transfusion candidates. Group and typing of expectant mothers and newborns may indicate the potential for ABO hemolytic disease of the fetus/newborn. Rh typing is used to determine Rh immune globulin candidacy for prenatal and postpartum patients.

Limitations

Abnormal plasma proteins, cold autoagglutinins, positive direct antiglobulin test, and, in some cases, bacteremia may interfere with test interpretation. Variable testing results may be observed with partial antigens, which are rare variants of the D antigen.

Methodology:

Hemagglutination

Performed:

TAT 1-2 days