ABO Grouping and Rho(D) Typing

CPT Code(s):

86900, 86901




Monday - Friday




This test is used in the determination of ABO blood group and Rh type for transfusion candidates. Group and typing of expectant mothers and newborns may indicate the potential for ABO hemolytic disease of the fetus/newborn. Rh typing is used to determine Rh immune globulin candidacy for prenatal and postpartum patients.


Abnormal plasma proteins, cold autoagglutinins, positive direct antiglobulin test, and, in some cases, bacteremia may interfere with test interpretation. Variable testing results may be observed with partial antigens, which are rare variants of the D antigen.

Specimen Requirements:

Special Instructions: If a CBC and/or an antibody screening test is ordered with this test, submit a separate 4 mL specimen for each test.

Type: Whole blood

Container/Tube: Lavender-top tube (EDTA)
  • Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze red cells.

Sample Volume: 4 mL

Minimum Volume: 1 mL (Repeat testing is not possible with this specimen volume.)

Rejection Criteria:

  • Gross hemolysis
  • Bacterial or other contamination

Collection Procedure:


Reference Values:


Interpretation Data:



UFHPL Test #: 68000

UFHPL Epic order code: LAB895


  • Fung MK, Grossman JB, Hillyer CD, Westhoff CM, eds. AABB Technical Manual. 18th ed. 2014.

Reported Notes: