Albumin (ALB)

CPT Code(s):



In 1965, Rodkey introduced a convenient, direct method for determining albumin concentrations in serum utilizing a neutral buffered solution of bromocresol green (BCG) as the dye binding indicator. In 1971, Doumas et al. increased the sensitivity of the reaction by adding a nonionic surfactant to the reagent to prevent turbidity and improve linearity. This Albumin method is a modification of the Doumas and Rodkey procedures utilizing a different buffering system. At pH 4.2, bromocresol green reacts with albumin to form an intense green complex. The absorbance of the albumin-BCG complex is measured bichromatically (600/800nm) and is proportional to the albumin concentration in the sample.


Monday - Friday


Within 24 hours



Specimen Requirements:

Type: Peripheral blood


  • Serum, gel
  • Plasma, gel

Sample Volume: 0.5mL serum; sshueparate serum or plasma from cells within two hours of collection.

Stability (collection to time of analysis/testing):

  • Ambient: 7 days
  • Refrigerated: 30 days
Unacceptable Conditions: Moderate-to-gross hemolysis

Collection Procedure:


Reference Values:

3.5 - 5.7 g/dL

Interpretation Data:



UFHPL Test #: 20015

UFHPL Epic order code: LAB45

Reported Notes: