BRAF Mutation Detection
Epic order code: LAB21083401
Polymerase chain reaction/DNA pyrosequencing
Monday - Friday
5 - 10 business days
BRAF mutation can be utilized as an independent predictor for colorectal cancer patients’ responsiveness to anti-epidermal growth factor receptor therapy. In addition to being used in MSI-high patients as an exclusion factor of HNPCC, the test can also guide therapy for melanoma patients (anti-BRAF).
Paraffin-embedded, formalin-fixed tissue block; in case the blocks are not available, 5 unstained slides are accepted. Ship the specimen at 20° - 25° C. Tissues fixed in formalin substitute are unacceptable.
Fine-needle biopsy, shipped frozen or on ice with RPMI-1640.
No mutation detected; interpretative report
- Mutation Detected: A mutation was detected in BRAF codon 600 (c. 1799 T > A).
- No Mutation Detected: No BRAF mutation was identified in the provided specimen of the patient codon 600 (c. 1799 T > A).
- QNS: Due to an inadequate specimen or less than 50% tumor cells present in the tissue