Direct Bilirubin

CPT Code(s):

82248

Methodology:

This direct bilirubin reagent utilizes a variation of the classic method developed by Van den Bergh and Mueller. Direct (conjugated) bilirubin couples directly with a diazonium salt of 3,5-dichloroaniline (DPD) in an acid medium to form azobilirubin. The direct bilirubin in serum is directly proportional to the color development of azobilirubin, which is measured bichromatically at 570/660 nm.

Performed:

Monday - Friday

Reported:

Within 24 hours

Use:

-

Specimen Requirements:

Type: Peripheral Blood

Container/Tube:

  • Serum, gel
  • Plasma, gel

Sample Volume: 0.5mL serum

Stability (collection to initiation of testing): Specimens should be protected from light.

  • Refrigerated: 3 days
  • Frozen: 3 months
Unacceptable Conditions:

  • Moderate or gross hemolysis

Collection Procedure:

Serum gel tubes should be centrifuged within 2 hours of collection.

Reference Values:

-

Interpretation Data:

-

Notes:

UFHPL Test #: 82248

UFHPL Epic order code: LAB52

Reported Notes:

-

Result:

-