FISH Screen: Prenatal Aneuploidy (Amniotic Fluid Only)

CPT Code(s):

  • 88271 x 5
  • 88274
  • 88291


Interphase nuclei fluorescence in-situ hybridization (FISH) analysis for 13, 18, 21, X/Y aneuploidy; requires concurrent classic cytogenetic analysis; not for standalone use


Monday - Saturday


10 - 14 days


This screening test is only used to detect the presence of common forms of aneuploidy fetal conditions, which include trisomies 21, 18 and 13, as well as various sex chromosome aneuploidies.

Specimen Requirements:

Amniotic Fluid: Aseptically collect approximately 1 cc of per week of gestational age in two sterile 15 mL conical centrifuge tubes and sequentially label the tubes. The initial 1 - 2 cc should not be included for chromosome studies (but may be utilized for AFP testing).

Do not freeze or fix samples in any manner contrary to the maintenance of cell viability. Maintain sample sterility; keep all specimens at room temperature; and deliver samples to the laboratory within 24 - 48 hours. Ship specimens at room temperature for overnight delivery.

Contact UF Health Pathology Laboratories prior to submission (approved by consultation).

Collection Procedure:


Reference Values:

By report; interpretive

Interpretation Data:



UFHPL Test #: 30065

Reported Notes: