FISH Study: Neoplastic Interphase CBFB

CPT Code(s):

  • 88271
  • 88275
  • 88291

Methodology:

Interphase nuclei fluorescence in-situ hybridization (FISH) break-apart rearrangement analyses

Performed:

Monday - Saturday

Reported:

5 - 12 days (subject to a broad range of variables)

Use:

This test is used to detect rearrangements involving the CBFB gene locus (16q22), which are most commonly associated with chromosome 16 rearrangements and the AML subtype M4eo.

Specimen Requirements:

  • Bone Marrow Aspirate: Obtain 1 - 2 cc of in a green-top sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
  • Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
  • Peripheral Blood Specimens: Peripheral blood specimens may be submitted in those clinical conditions (i.e. circulating blasts).
Unacceptable Conditions:
  • Frozen specimens
  • Specimens for FISH study only > one week old
  • Specimens for concurrent FISH and cytogenetic studies > 48 hours old*
  • Formalin, alcohol or paraffin-preserved specimens
Stability (collection to initiation of testing):
  • Ambient: 24 - 48 hours*
  • Frozen: Unacceptable

* Cell viability of specimens requiring cell culture may be compromised in specimens more than 48 hours old upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.

Do not freeze or fix samples in any manner contrary to the maintenance of cell viability. Maintain sample sterility; keep all specimens at room temperature; and deliver samples to the laboratory within 24 - 48 hours. Ship specimens at room temperature for overnight delivery.

Collection Procedure:

-

Reference Values:

By report; interpretive

Interpretation Data:

-

Notes:

UFHPL Test #: 30105

Reported Notes:

-

Result:

Interpretive report