FISH Study: Neoplastic Interphase ETV6

CPT Code(s):

  • 88271
  • 88275
  • 88291

Methodology:

Interphase nuclei fluorescence in-situ hybridization (FISH) break-apart rearrangement analyses; a concurrent or previous conventional cytogenetic workup is required/recommended (where possible).

Performed:

Monday - Saturday

Reported:

5 - 12 days

Use:

This test is used to detect cryptic rearrangements involving the ETV6 gene locus, which have been implicated in broad range of hematological conditions including acute leukemias and MDS.

Specimen Requirements:

  • Bone Marrow Aspirate: Obtain 1 - 2 cc of in a green-top sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
  • Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
  • Peripheral Blood Specimens: Peripheral blood specimens may be submitted in those clinical conditions (i.e. circulating blasts) and/or circumstances (where warranted).
  • Unacceptable Conditions:
    • Frozen specimens
    • Specimens for FISH study only greater than one week old
    • Specimens for concurrent FISH and cytogenetic studies greater than 48 hours old*
    • Formalin, alcohol or paraffin-preserved specimens
  • Stability (collection to initiation of testing):
    • Ambient: 24 - 48 hours*
    • Frozen: Unacceptable

* Cell viability of specimens requiring cell culture may be compromised in specimens more than 48 hours old upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.

Collection Procedure:

-

Reference Values:

By report; interpretive

Interpretation Data:

-

Notes:

UFHPL Test #: 30160

Reported Notes:

-

Result:

-