FLT3 Gene Mutation Analysis

CPT Code(s):

81245, 81246

Methodology:

Multiplex polymerase chain reaction (PCR) with capillary electrophoresis

Performed:

Monday - Friday

Reported:

Within 7 days

Use:

The FLT3 gene encodes a cell surface receptor tyrosine kinase that is expressed on early hematopoietic stem cells. Activating mutations in FLT3 occur in approximately 25 to 30 percent of patients with acute myelogenous leukemia (AML). The mutation generally occurs as either an internal tandem duplication (ITD) within the juxtamembrane domain, or as a missense point mutation within the tyrosine kinase domain (TKD) at codon 835. This test detects both the FLT3-ITD and FLT3-TKD (codon 835) mutations. The presence of a FLT3-ITD mutation has been associated with a significantly poorer outcome in normal karyotype AML.

Specimen Requirements:

Collect blood or bone marrow in a lavender-top (EDTA) tube. Refrigerate and send intact specimen(s) within 48 hours of collection.

Unacceptable Conditions:

  • Paraffin block specimens

Collection Procedure:

-

Reference Values:

-

Interpretation Data:

-

Notes:

UFHPL Test #: 10082

Reported Notes:

-

Result:

-