Human Papillomavirus (HPV) Detection, High Risk with Genotyping

CPT Code(s):

87624

Methodology:

Polymerase chain reaction amplification with real-time detection using Cobas 4800

Performed:

Monday, Wednesday and Friday

Reported:

4 days from receipt of the specimen

Use:

  • Detecting the presence of high-risk HPV DNA and determining the specific genotype (HPV 16, HPV 18 or other high-risk HPV (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68)
  • This test helps fulfill the new ACS, ASCCP and ASCP screening guidelines for Pap tests.

Specimen Requirements:

Specimens in ThinPrep® Cytyc PreservCyt® Solution:There must be at least 1 mL of PreservCyt® solution remaining for the result.

Remarks:Vaginal specimens are not preferred.

Unacceptable Conditions:

  • Frozen specimens
  • Swabs or samples in any other transport media
Stability (collection to initiation of testing):

  • Ambient:
    • Thin Prep® or PreservCyt®: Three weeks at 15° - 30° C

Reference Values:

Negative (interpretative report provided)

Interpretation Data:

Results

HPV High-Risk Result with Genotyping:

  • HPV 16 DNA: Result
  • HPV 18 DNA: Result
  • High-Risk HPV DNA (non-16,18): Result
Result Interpretation
Positive (detected) Corresponding HPV
was detected.
Negative (not detected) Corresponding HPV
was not detected.
Invalid A valid result
was not obtained.

A specimen will be reported as quantity not sufficient for testing (QNS) if there is less than 1 mL of specimen in the submitted liquid Pap test vial.