Luteinizing Hormone (LH)

CPT Code(s):



Chemiluminescent immunometric assay


Monday - Friday


Within 3 days


Luteinizing hormone measurements are used to define the hypothalamic-pituitary-gonadal axis. Serum gonadotropin determinations permit distinguishing between primary gonadal failure and deficient gonadal stimulation. If LH and follicle-stimulating hormone levels are elevated, primary gonadal failure is present, whereas if gonadotropin levels are low, deficient gonadal stimulation has resulted in the hypogonadal state. LH measurement is also clinically important because LH and growth hormone are frequently the first hormones to be affected by pituitary disease. Serum LH determinations have been very useful in the diagnosis and treatment of infertility in women. A midcycle rise is a good indication that ovulation will occur approximately 24 hours later. Subfertile couples, and women being treated withgonadotropins for infertility, can be informed that ovulation is imminent.

Specimen Requirements:

Collect blood by venipuncture into an SST/gold-top tube. For serum, allow the specimens to clot at room temperature. Separate the serums from the cells, using a refrigerated centrifuge and avoid hemolysis. If possible, keep specimens cold (2 - 8°C).

Stability (collection to time of analysis/testing):

  • Refrigerated (2 - 8°C): 14 days
  • Frozen (-20°C): 2 months

Collection Procedure:


Reference Values:

  • Adult Males (≥ 18 years): 0.8 to 7.6 mIU/mL
  • Adult Females (≥ 18 years):
    • Postmenopausal: 11.3 - 39.8 mIU/mL
    • Oral Contraceptives: ND - 8.0 mIU/mL
  • Ovulatory Cycles:
    • Follicular Phase: 1.1 - 11.6 mIU/mL
    • Midcycle: 17 - 77 mIU/mL
    • Luteal Phase: ND - 14.7 mIU/mL
    • Perimenstrual (± 8 days): ND - 12.0 mIU/mL

Interpretation Data:



UFHPL Test #: 20325

Reported Notes: