Ova and Parasites Examination
UFHPL Epic order code: LAB2010
Formalin concentrate and trichome stain
Monday - Friday
This test is used to establish a diagnosis of parasitic infestation. It includes the concentration of material and examination of specimen for ova and parasites by conventional iodine/saline and trichrome staining. This test will not detect Cryptosporidium, Cyclospora cayetanensis or Microsporidium.
One negative result does not rule out the possibility of parasitic infestation. Stool examination for Giardia may be negative in early stages of infection, in patients who shed organisms cyclically, and in chronic infections.1 The sensitivity of microscopic methods for the detection of Giardia range from 46% to 95%.2 Tests for Giardia antigen may have a higher yield.3
Special Instructions: Include any pertinent clinical and travel history on the test requisition form.
Patient Preparation: Usual aseptic technique
Type: Sputum or feces
Sample Volume: 3 to 4 mL sputum or 5 mL feces
Minimum Volume: 3 mL
Storage: Store specimens at room temperature.
- Because parasite morphology will not be preserved, specimens sent on diaper or tissue paper are not acceptable to the laboratory because of risk to lab personnel.
- Grossly leaking specimens may not be processed.
- Specimen containing interfering substances (eg, castor oil, bismuth, Metamucil®, barium specimens delayed in transit and those contaminated with urine) will not have optimal yield.
- Unlabeled specimen or name discrepancy between specimen and test request label; expired transport device
- Specimen not received in O & P preservative transport containers with formalin and PVA
- Sputum: If paragonimiasis or echinococcosis is suspected, submit specimen in 10 percent formalin.
- Feces: Submit specimens in a parasite preservative kit. Inoculate both the PVA and the formalin tubes. Fresh feces should not be submitted. State the preliminary diagnosis.
- Other: Contact the laboratory at 888.375.LABS (5227) for specific instructions.
- All: Multiple specimens may be necessary to recover ova or trophozoites. Three specimens are recommended (each is charged).
- Submit one or two specimens per diarrheal illness immediately.
- If those are negative, submit an additional specimen after five days.
- Patients who are immunocompromised by AIDS, malignancy, or immunosuppressive therapy may require additional testing for unusual stool pathogens.