Uric Acid, Blood

Methodology: 
Uric acid can be determined by direct measurement, by measurement of oxygen consumed or by measurement ofhydrogen peroxide produced by the uricase reaction. Several methods utilize coupled enzyme reactions to detect the hydrogen peroxide produced by the uricase reaction. Fossati et al1 proposed a method which utilized the well established Trinder reaction to measure the hydrogen peroxide produced, and a substituted phenol to enhance sensitivity. This Uric Acid procedure is a modifcation of the Fossati method. Uric acid is converted by uricase to allantoin and hydrogen peroxide. Hydrogen peroxide reacts with 4-aminoantipyrine (4-AAP) in the presence of N,N-bis(4-sulfobutyl)-3,5-dimethylaniline, disodium salt (MADB) to produce a chromophore which is read bichromatically at 660/800 nm. The amount of dye formed is proportional to the uric acid concentration in the sample.
Performed: 
Monday - Friday
Reported: 
Within 24 hours
Purpose and principle: 

Measurements of uric acid are used in the diagnosis and treatment of numerous renal and metabolic disorders, including renal failure, gout, leukemia, psoriasis, starvation or other wasting conditions, and for patients receiving cytotoxic drugs.

Specimen Requirements: 

Type: Peripheral blood

Container/Tube:
  • Serum, gel
  • Plasma, gel

Sample Volume: 0.5 mL

Stability (collection to time of analysis/testing):
  • Refrigerated: 3 – 5 days
  • Frozen: 6 months
Unacceptable Conditions: Moderate-to-gross hemolysis
Reference Values: 
  • Female: 2.3 – 6.6 mg/dL
  • Male: 4.4 - 7.6 mg/dL
CPT Code (s): 
84550
Notes: 

UFHPL Test #: 20500

UFHPL Epic order code: LAB141

Collection procedure: 
Serum gel tubes should be centrifuged within 2 hours of collection.
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