UFHPL Epic order code: LAB112
Container/Tube: Serum gel or heparinized plasma
Sample Volume: Submit full tubes.
Minimum volume: 0.5 mL serum or plasma
- Send specimens in their original tubes.
- If there is a delay in transport of >1 hour, centrifuge tube.
Storage: Samples are stable for up to 4 days at 2 – 8ºC.
Alkaline phosphatase activity is determined by measuring the rate of conversion of p-nitro-phenylphosphate (pNPP) in the presence of 2-amino-2-methyl-1-propanol (AMP) at pH 10.4. The rate of change in absorbance due to the formation of pNP is measured bichromatically at 410/480 nm and is directly proportional to the ALP activity in the sample. (Package Insert, OSR General Chemistry, BAOSR6x04.03, 2014-04)
Within 24 hours
Adult Females 33-133 U/L
Monday - Friday