Amylase

Additional Information:

UFHPL Epic order code: LAB48

CPT Code(s):

82150

Specimen Requirements:

Type: Serum

Container/Tube: Serum gel

Sample volume: Submit full tube

Minimum volume: 0.5 mL serum

Preparation:

  1. Send specimen in original tube.
  2. If there is a delay in transport of more than 1 hour, centrifuge the tube.
Stability (collection to shipping of the sample):

  • Ambient (15 - 25°C): 7 days
  • Refrigerated (2 – 8ºC): 30 days

Unacceptable Conditions: Samples with moderate or marked hemolysis will be rejected.

Specimen Retention Time: 5 days

Use:

Amylase is found primarily in the pancreas and salivary glands. Elevated serum levels are associated with acute pancreatitis and other pancreatic disorders as well as mumps and bacterial parotitis.

Methodology:

This Amylase procedure utilizes 2-chloro-4-nitrophenyl-a-D-maltotrioside (CNPG3) as substrate.2 This substrate reacts directly with a-Amylase and does not require the presence of ancillary enzymes. The release of 2-chloro-4-nitrophenol (CNP) from the substrate and the resulting absorbance increase per minute is directly related to the a-Amylase activity in the sample. The resulting increase in absorbance can be measured spectrophotometrically at 410/480nm. (Package Insert: OSR General Chemistry BAOSR6x06.02; 2012-01)

Reported:

Within 1 day

Reference Values:

Serum: 29 - 103 U/L

Performed:

Monday - Friday