Additional Information:
UFHPL Epic order code: LAB48
CPT Code(s):
82150
Specimen Requirements:
Type: Serum
Sample volume: Submit full tube
Minimum volume: 0.5 mL serum
Preparation:
- Send specimen in original tube.
- If there is a delay in transport of more than 1 hour, centrifuge the tube.
- Ambient (15 – 25°C): 7 days
- Refrigerated (2 – 8ºC): 30 days
Unacceptable Conditions: Samples with moderate or marked hemolysis will be rejected.
Specimen Retention Time: 5 days
Use:
Amylase is found primarily in the pancreas and salivary glands. Elevated serum levels are associated with acute pancreatitis and other pancreatic disorders as well as mumps and bacterial parotitis.
Methodology:
This Amylase procedure utilizes 2-chloro-4-nitrophenyl-a-D-maltotrioside (CNPG3) as substrate.2 This substrate reacts directly with a-Amylase and does not require the presence of ancillary enzymes. The release of 2-chloro-4-nitrophenol (CNP) from the substrate and the resulting absorbance increase per minute is directly related to the a-Amylase activity in the sample. The resulting increase in absorbance can be measured spectrophotometrically at 410/480nm. (Package Insert: OSR General Chemistry BAOSR6x06.02; 2012-01)
Reported:
Within 1 day
Reference Values:
Serum: 29 – 103 U/L