BRAF Mutation Detection

Additional Information:

Epic order code: LAB21083401

CPT Code(s):

  • 81210
  • 88381

Specimen Requirements:

Paraffin-embedded, formalin-fixed tissue block; in case the blocks are not available, 5 unstained slides are accepted. Ship the specimen at 20° - 25° C. Tissues fixed in formalin substitute are unacceptable.

Fine-needle biopsy, shipped frozen or on ice with RPMI-1640.


BRAF mutation can be utilized as an independent predictor for colorectal cancer patients’ responsiveness to anti-epidermal growth factor receptor therapy. In addition to being used in MSI-high patients as an exclusion factor of HNPCC, the test can also guide therapy for melanoma patients (anti-BRAF).


Polymerase chain reaction/DNA pyrosequencing


5 - 10 business days

Reference Values:

No mutation detected; interpretative report


Monday - Friday

Interpretation Data:


  • Mutation Detected: A mutation was detected in BRAF codon 600 (c. 1799 T > A).
  • No Mutation Detected: No BRAF mutation was identified in the provided specimen of the patient codon 600 (c. 1799 T > A).
  • QNS: Due to an inadequate specimen or less than 50% tumor cells present in the tissue