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CD4:CD8 Ratio Profile

Additional Information:

UFHPL Epic order code: LAB4477

HIV-1 infection results in a decrease of CD4 T cells, an increase of CD8 T cells, a decrease in the CD4:CD8 ratio, and a progressive destruction of immune function. In HIV-1 seropositive patients, enumeration of CD4 T cells may be used for prognostic purposes and to monitor disease progression and antiretroviral therapy.

References

  • Centers for Disease Control and Prevention (CDC). 1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV). MMWR. 1997 Jan 10; 46:RR-2.
  • Giorgi J, Kesson A, Chou CC. Immunodeficiency and infectious diseases. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 25.
  • Stevens RA, Lempicki RA, Natarajan V, et al. General immunologic evaluation of patients with immunodeficiency virus infection. In: Detrick B, Hamilton RG, Folds JD, et al, eds. Manual of Molecular and Clinical Laboratory Immunology. 7th ed. Washington, DC: ASM Press;2006:chap 95.

CPT Code(s):

86360

Specimen Requirements:

Type: Whole blood

Container/Tube: Lavender-top tube (EDTA) and yellow-top tube (ACD-A) or (ACD-B)
  • Invert each tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimens so they will arrive at the laboratory within 48 hours of collection. Indicate the date and time of venipuncture on the tubes and on the test requisition.

Sample Volume: Fill the tubes to capacity.

Storage: Store specimens at room temperaturen.

Stability (collection to time of analysis/testing):

  • Ambient: 2 days
  • Refrigerated: Unstable
  • Frozen: Unstable
  • Freeze/Thaw Cycles: Unstable
Rejection Criteria:

  • Hemolysis
  • Specimen refrigerated or frozen
  • Clotted specimen
  • Contaminated specimen

Use:

This test includes:

  • Percentage CD4+
  • Absolute CD4+
  • Percentage CD8+
  • Absolute CD8+
  • CD4:CD8 ratio
  • CBC with differential

Methodology:

Flow cytometry

Reference Values:

Reference Intervals

95 Percent Confidence Interval
CD Age Percentage Minimum Percentage Maximum Cells/mm3 Minumum Cells/mm3 Maximum
Pediatric reference intervals are from Comans-Bitter WM, de Groot R, van den Beemd R, et al. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatrics. 1997 Mar; 130(3):388-393.
CD4+ Neonates 17 52 400 3500
1 week to
2 months old		 41 68 1700 5300
2 − 5
months old		 33 58 1500 5000
5 − 9
months old		 33 58 1400 5100
9 − 15
months old		 31 54 1000 4600
15 − 24
months old		 25 50 900 5500
2 − 5
years old		 23 48 500 2400
5 − 10
years old		 27 53 300 2000
10 − 16
years old		 25 48 400 2100
Adults 31 59 359 1519
CD8+ Neonates 10 41 200 1900
1 week to
2 months old		 9 23 400 1700
2 − 5
months old		 11 25 500 1600
5 − 9
months old		 13 26 600 2200
9 − 15
months old		 12 28 400 2100
15 − 24
months old		 11 32 400 2300
2 − 5
years old		 14 33 300 1600
5 − 10
years old		 19 34 300 1800
10 − 16
years old		 9 35 200 1200
Adults 12 36 109 897
T4:T8 ratio Adults 0.92 3.72

Reported:

3 – 5 days