Additional Information:
UFHPL Epic order code: LAB4477
HIV-1 infection results in a decrease of CD4 T cells, an increase of CD8 T cells, a decrease in the CD4:CD8 ratio, and a progressive destruction of immune function. In HIV-1 seropositive patients, enumeration of CD4 T cells may be used for prognostic purposes and to monitor disease progression and antiretroviral therapy.
References
- Centers for Disease Control and Prevention (CDC). 1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV). MMWR. 1997 Jan 10; 46:RR-2.
- Giorgi J, Kesson A, Chou CC. Immunodeficiency and infectious diseases. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 25.
- Stevens RA, Lempicki RA, Natarajan V, et al. General immunologic evaluation of patients with immunodeficiency virus infection. In: Detrick B, Hamilton RG, Folds JD, et al, eds. Manual of Molecular and Clinical Laboratory Immunology. 7th ed. Washington, DC: ASM Press;2006:chap 95.
CPT Code(s):
86360
Specimen Requirements:
Type: Whole blood
Container/Tube: Lavender-top tube (EDTA) and yellow-top tube (ACD-A) or (ACD-B)
- Invert each tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimens so they will arrive at the laboratory within 48 hours of collection. Indicate the date and time of venipuncture on the tubes and on the test requisition.
Sample Volume: Fill the tubes to capacity.
Storage: Store specimens at room temperaturen.
Stability (collection to time of analysis/testing):
- Ambient: 2 days
- Refrigerated: Unstable
- Frozen: Unstable
- Freeze/Thaw Cycles: Unstable
Rejection Criteria:
- Hemolysis
- Specimen refrigerated or frozen
- Clotted specimen
- Contaminated specimen
Use:
This test includes:
- Percentage CD4+
- Absolute CD4+
- Percentage CD8+
- Absolute CD8+
- CD4:CD8 ratio
- CBC with differential
Methodology:
Flow cytometry
Reference Values:
Reference Intervals
95 Percent Confidence Interval | |||||
---|---|---|---|---|---|
CD | Age | Percentage Minimum | Percentage Maximum | Cells/mm3 Minumum | Cells/mm3 Maximum |
Pediatric reference intervals are from Comans-Bitter WM, de Groot R, van den Beemd R, et al. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatrics. 1997 Mar; 130(3):388-393. | |||||
CD4+ | Neonates | 17 | 52 | 400 | 3500 |
1 week to 2 months old |
41 | 68 | 1700 | 5300 | |
2 − 5 months old |
33 | 58 | 1500 | 5000 | |
5 − 9 months old |
33 | 58 | 1400 | 5100 | |
9 − 15 months old |
31 | 54 | 1000 | 4600 | |
15 − 24 months old |
25 | 50 | 900 | 5500 | |
2 − 5 years old |
23 | 48 | 500 | 2400 | |
5 − 10 years old |
27 | 53 | 300 | 2000 | |
10 − 16 years old |
25 | 48 | 400 | 2100 | |
Adults | 31 | 59 | 359 | 1519 | |
CD8+ | Neonates | 10 | 41 | 200 | 1900 |
1 week to 2 months old |
9 | 23 | 400 | 1700 | |
2 − 5 months old |
11 | 25 | 500 | 1600 | |
5 − 9 months old |
13 | 26 | 600 | 2200 | |
9 − 15 months old |
12 | 28 | 400 | 2100 | |
15 − 24 months old |
11 | 32 | 400 | 2300 | |
2 − 5 years old |
14 | 33 | 300 | 1600 | |
5 − 10 years old |
19 | 34 | 300 | 1800 | |
10 − 16 years old |
9 | 35 | 200 | 1200 | |
Adults | 12 | 36 | 109 | 897 | |
T4:T8 ratio | Adults | 0.92 | 3.72 |
Reported:
3 – 5 days