Cytopathology: Breast Nipple Secretion

CPT Code(s):


Specimen Requirements:

Supplies: You will need two clean glass slides (single-end frosted), fixative (95% ethanol) and a request form. Collect cells in nipple discharge. Collect 1 – 2 drops only on the center on one of the labeled slides. Place the other labeled slide face down on the other and let the specimen slowly spread out between the two slides. Working quickly, separate the two slides like opening a book, resulting in a mirrored-image specimen. Do not press the two slides together. Immediately fix one slide in 95% ETOH.

Storage/Transport Temperature: Submit specimens according to Biological Substance, Category B, shipping guidelines.

Unacceptable Conditions: Frozen, sterile container > 2 hours old without heparin

Stability (collection to initiation of testing):

  • Fixed Slides: Indefinitely
  • Ambient: Indefinitely
  • Frozen: Unacceptable


This test is used to aid in the diagnosis of breast malignancy.




1 – 2 days