CPT Code(s):
88182, 88342
Specimen Requirements:
Paraffin block
Storage/Transport: Ship specimens at 20° – 25° C to protect them from excessive heat. Include a surgical pathology report.
Unacceptable Specimens:
- Specimens that do not contain placental villi with trophoblast
- Specimens in fixatives other than 10% neutral-buffered formalin or zinc formalin
- Specimens in Bouin, B5, Omni or Carnoy’s Solution
Stability (collection to initiation of testing):
- Ambient: Formalin-fixed paraffin block
Antibody: p57Kip2
Detection Method: EnVision + Dual Link System HRP
Use:
This test is used to separate cells with different traits from a mixed-cell population. Assays are performed on paraffin-embedded, formalin-fixed tissue. Flow cytometry, in combination with p57 staining, can be used to differentiate between partial moles, complete moles or hydropic changes. Partial moles are usually triploid and express p57; complete moles are usually diploid or tetraploid and do not express p57, while hydropic changes are usually diploid and do not express p57 (Adv Anat Pathol. 2005: 12; 116-125).
Methodology:
Flow cytometry and immunohistochemistry staining with p57 (FISH available spring 2011; call for availability)
Reported:
7 days