FISH Panels

Additional Information:

UFHPL Epic order code: LAB5851

For each required FISH analysis, select the entire panel or customize your own panel.
If the probe is unspecified, call UF Health Pathology Laboratories at 888.375.LABS (5227) for availability.

*Please note:  If ordering both a chromosome analysis/FISH study AND a microarray analysis, please draw two separate tubes of blood. Chromosomes/FISH studies require as close to 2.0ml+ as possible, collected in a dark green sodium heparin tube. Microarrays require the same volume (~2.0ml+) and the preferred tube is a lavender EDTA, but a dark green sodium heparin is also acceptable. Further details for UF PathLab’s cytogenetics requirements are available here and LabCorp microarray requirements are available here.

 

FISH Panels: 7-14 days

Myelodysplastic syndrome (MDS)

EGR1 (del 5q/mono 5)

D7S522 (del 7q/mono 7)

D8Z2

D20S108 (del 20q)

Acute myeloid leukemia (AML)

RUNX1T1/RUNX1 [t(8;21)]

BCR/ABL1 [t(9;22)]

KMT2A 

CBFB::MYH11

TP53

AML COG

RUNX1T1/RUNX1 [t(8;21)]

KMT2A
CBFB::MYH11

Acute lymphoid leukemia (ALL) COG standard risk

BCR/ABL1 [t(9;22)]

KMT2A

ETV6/RUNX1 [t(12;21)]

CEP 4/CEP 10

ALL COG high risk 

Std risk PLUS:

ABL2

PDGFRB

ABL1

ALL pediatric non-COG

TCF3/PBX1 [t(1;19)]

CKDN2A (del 9p)

BCR/ABL1 [t(9;22)]

KMT2A

ETV6/RUNX1 [t(12;21)]

CEP 4/CEP 10

ALL adult

TCF3/PBX1 [t(1;19)]

BCR/ABL1 [t(9;22)]

KMT2A

ETV6/RUNX1 [t(12;21)]

Plasma cell neoplasm (PCN) or multiple myeloma (MM)

CKS1B (1q)
Chromosome 5-9-15 Aueuploidy

D13S319 (del 13q/mono 13)

IGH

TP53

Chronic lymphocytic leukemia (CLL)

ATM (del 11q)

D12Z3

D13S319 (del 13q/mono 13)

TP53

Myeloproliferative neoplasm (MPN) w/ eosinophilia

BCR/ABL1 [t(9;22)]

ETV6

PDGFRb

NHL

BCL6

MYC

IGH/CCND1 [t(11;14)]

BCL2 

T-ALL or mixed lineage leukemia

BCR/ABL1 [t(9;22)]

KMT2A 

FISH Study: APL STAT FISH for t(15;17) PML::RARA: 24-28 hours

FISH Study: 22q11.2 Microdeletion Syndrome (DiGeorge/VCF): 7-21 days

FISH Study: SRY Sex Determination (Yp11.3):  7-21 days

FISH Study: X/Y Sex Chromosome Determination:  7-21 days

Methodology:

Fluorescence in-situ hybridization (FISH)