FISH Study: Neoplastic Interphase ALK

CPT Code(s):

  • 88271
  • 88275
  • 88291

Specimen Requirements:

  • Lymphatic Tissues: Aseptically obtain a sample of approximately 2mm x 2mm x 2mm and deposit it in tissue culture medium.
  • Bone Marrow Aspirate: Obtain 1 - 2 cc of aspirate in a green-top sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
  • Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
  • Peripheral Blood Specimens: Peripheral blood specimens may be submitted in certain clinical conditions (e.g. circulating lymphoma cells).

Do not freeze or fix samples in any manner contrary to the maintenance of cell viability. Maintain sample sterility; keep all specimens at room temperature; and deliver samples to the laboratory within 24 - 48 hours. Ship specimens at room temperature for overnight delivery.

Unacceptable Conditions:
  • Frozen specimens
  • Specimens for FISH study only greater than one week old
  • Specimens for concurrent FISH and cytogenetic studies greater than 48 hours old*
  • Formalin, alcohol or paraffin-preserved specimens
Stability (collection to initiation of testing):
  • Ambient: 24 - 48 hours*
  • Frozen: Unacceptable

* Cell viability of specimens requiring cell culture may be compromised in specimens older than 48 hours upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.


Interphase nuclei fluorescence in-situ hybridization (FISH) break-apart rearrangement analyses; a concurrent or previous conventional cytogenetic workup is required/recommended (where possible).


5 - 12 days

Reference Values:

By report, interpretive


Monday - Saturday