FISH Study: Neoplastic Interphase BCL6
- Lymphatic Tissues:Aseptically obtain a sample of approximately 2mm x 2mm x 2mm and deposit in tissue culture medium*.
- Bone Marrow Aspirate: Obtain 1 - 2 cc of in a green-top sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
- Bone Marrow Core Biopsy:Deposit samples in a steBy report; interpretiverile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
- Peripheral Blood Specimens:Peripheral blood specimens may be submitted in those clinical conditions (i.e. circulating blasts) and/or circumstances (where warranted).
Do not freeze or fix samples in any manner contrary to the maintenance of cell viability. Maintain sample sterility; keep all specimens at room temperature; and deliver samples to the laboratory within 24 - 48 hours. Ship specimens at room temperature for overnight delivery.
- Unacceptable Conditions:
- Frozen specimens
- Specimens for FISH study only greater than one week old
- Specimens for concurrent FISH and cytogenetic studies greater than 48 hours old*
- Formalin, alcohol or paraffin-preserved specimens
- Stability (collection to initiation of testing):
- Ambient: 24 - 48 hours*
- Frozen: Unacceptable
* Cell viability of specimens requiring cell culture may be compromised in specimens more than 48 hours old upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.
This test is used to detect rearrangements involving the BCL6 gene locus (3q27), which are most commonly associated with B-cell NHL (follicular lymphoma, diffuse large B-cell lymphoma). These rearrangements are also seen in nodular lymphocyte predominant Hodgkin lymphomas.
Interphase nuclei fluorescence in-situ hybridization (FISH) break-apart rearrangement analyses; a concurrent or previous conventional cytogenetic workup is required/recommended (where possible).
5 - 12 days
By report; interpretive
Monday - Saturday