FISH Study: CDKN2A with 9cen

Additional Information:

UFHPL Epic order code: LAB5851

CPT Code(s):

  • 88271
  • 88275
  • 88291

Specimen Requirements:

  • Bone Marrow Aspirate: Obtain 1 – 2 cc of in a green-top (sodium heparin) tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
  • Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
  • Peripheral Blood Specimens: Peripheral blood specimens may be submitted in those clinical conditions (i.e. circulating blasts) and/or circumstances (where clinically warranted).

* Cell viability of specimens requiring cell culture may be compromised in specimens more than 48 hours old upon receipt. Refrigeration at 2° – 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.

Unacceptable Conditions:

  • Frozen specimens
  • Specimens more than a week old for FISH study only
  • Specimens for concurrent FISH and cytogenetic studies more than 48 hours old*
  • Formalin-, alcohol- or paraffin-preserved specimens

Stability (collection to initiation of testing):

  • Ambient: 24 – 48 hours*
  • Frozen: Unacceptable
Do not freeze or fix samples in any manner contrary to the maintenance of cell viability. Maintain sample sterility; keep all specimens at room temperature; and deliver samples to the laboratory within 48 hours. Ship specimens at room temperature for overnight delivery.


This test is used to detect the presence of chromosome 9 short-arm deletions, which include the CDKN2A gene locus and are observed in a broad-range of malignancies, including pediatric and adult ALL.


Interphase nuclei fluorescence in-situ hybridization (FISH) copy number analyses


Within 5 – 12 days

Reference Values:

By report; interpretive