FISH Study: Neoplastic Interphase D8Z2 (Trisomy 8)
UFHPL Test #: XXXXX
- Bone Marrow Aspirate: Obtain 1 - 2 cc of in a green-top sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
- Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
- Peripheral Blood Specimens: Peripheral blood specimens may be submitted in those clinical conditions (i.e. circulating blasts) and/or circumstances (where warranted).
- Unacceptable Conditions:
- Frozen specimens
- Specimens for FISH study only greater than one week old
- Specimens for concurrent FISH and cytogenetic studies greater than 48 hours old*
- Formalin, alcohol or paraffin-preserved specimens
- Stability (collection to initiation of testing):
- Ambient: 24 - 48 hours*
- Frozen: Unacceptable
* Cell viability of specimens requiring cell culture may be compromised in specimens more than 48 hours old upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.
This test is used to detect trisomy for chromosome 8, which is one of the most common chromosome abnormalities in myeloid disorders.
Interphase nuclei fluorescence in-situ hybridization (FISH) copy number analyses; a concurrent or previous conventional cytogenetic workup is required/recommended (where possible)
5 - 12 days
By report; interpretive
Monday - Saturday